Diagnostics
Seminested PCR-enzyme immunoassay is favorable method for candidemia diagnosis
Published in Blood Weekly, October 28th, 2004
According to a study from Kuwait, "We have developed a semi-nested PCR-enzyme immunoassay (snPCR-EIA) for the detection of Candida species in serum specimens, and the sensitivity of amplicon detection was compared with the detection of amplified product by agarose gel electrophoresis (AGE)."
"The universal outer primers amplified the 3' end of 5.8S and the 5' end of 28S rDNA including the internally transcribed spacer 2 (ITS2) in PCR with genomic DNA as template from all the tested Candida species."
"The biotin-labeled...
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Source: Blood Weekly (2004-10-28)
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