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Adenovirus Detection

Novel method detects and types adenoviruses directly from conjunctival scrapings

Published in Blood Weekly, November 11th, 2004

A novel method is used to detect and type adenoviruses directly from conjunctival scrapings.

In a recent report, researchers in Japan conducted a study "to develop a new method to detect and type adenoviruses directly from conjunctival scrapings using loop-mediated isothermal amplification (LAMP) with adenovirus (ad) type specific primer."

"Using primers specific for the gene of ad1, ad3, ad4, ad8, ad19 and ad37, heat denatured adenovirus DNA was amplified by the LAMP and polymerase chain reaction (PCR). Alkaline lysed adenovirus prototype and conjunctival scrapings were also used directly as templates," wrote T. Wakabayashi and colleagues.

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