Published in Blood Weekly, December 2nd, 2004
According to scientists in Germany, "To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness.
"Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and the type I heat-stable enterotoxin (ST 1) genes, using the LC hybridization probe format. A duplex assay for ST I with two...
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