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Papillomavirus

Nested PCR with PGMY09/11 and GP5+/6+ primer sets improves HPV DNA detection

Published in Blood Weekly, February 10th, 2005

Nested PCR with PGMY09/11 and GP5+/6+ primer sets improves human papillomavirus DNA detection.

According to recent research published in the Journal of Virological Methods, "Based on epidemiological and research evidence, human papillomavirus has a causal role in cervical carcinogenesis. Several HPV detection methods exist to date; the most commonly used method for detection of genital HPVs consists of nested PCR using the MY09/11 and GP5+/6+ primer sets (MY/GP+)."

"Recently," said A.L.F. Haws and coworkers, "the PGMY09/11 primer set, a modified version of the MY09/11 primer set, was introduced for single PCR and was found to detect a wider...

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