Published in Blood Weekly, May 5th, 2005
According to a study from England, "To date no reliable method has been developed for the isolation of RNA from cells seeded onto cylindrical vascular grafts.
"This study was performed in order to develop a reliable methodology for isolating RNA from cylindrical conduits made from poly(carbonate-urea)urethane (PU). Human umbilical vein EC were seeded onto PU vascular grafts and an Alamar blue assay performed to assess cell viability."
"Cells were prepared for RNA extraction by trypsinisation, cell scraping and direct application of cell lysis...
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