African Swine Fever
Invader assay adapted for African swine fever virus DNA detection
Published in Blood Weekly, May 5th, 2005
"A closed tube isothermal Invader assay (Third Wave Technologies Inc, Madison, Wisconsin, USA) was adapted for the detection of African swine fever virus (ASFV) DNA. Several ASFV Invader assays were designed successfully and tested on a real-time PCR instrument (iCycler, BioRad).
"The assay exhibiting the lowest signal/noise ratio (VP73 ASFV Invader Assay) was analyzed further using serial 10-fold dilutions of Lisbon 60 ASFV viral genome," researchers in North Ireland report.
"The assay sensitivity was determined to be in the order of 2500 copies of...
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Source: Blood Weekly (2005-05-05)
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