Stem Cell Research

Ocular surface cells contain ABCG2-dependent SP cells with stem cell features

Published in Blood Weekly, July 21st, 2005

Ocular surface epithelia contain ABCG2-dependent side population cells with stem cell features.

"When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). Stem cells from several tissues have been shown to possess the SP phenotype," researchers in the United States report.

"As the lack of specific surface markers has hindered the isolation and subsequent biochemical characterization of epithelial stem cells this study...

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Source: Blood Weekly (2005-07-21)

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