Hepatitis B Virus

Extraction of hepatitis B virus RNA and DNA automated for real-time PCR

Published in Blood Weekly, January 12th, 2006

Research from Belgium has chronicled the automated extraction of hepatitis B virus (HBV) RNA and DNA for high-throughput quantitative real-time PCR.

"The performance of the m1000 system (Abbott Laboratories, Illinois) as a front-end extraction system for high-throughput in-house quantitative real-time PCR assays was analyzed and compared to that of manual extraction of plasma and serum samples HCV and hepatitis B virus (HBV) and EDTA-blood samples (cytomegalovirus [CMV] and Epstein-Barr virus [EBV])."

K. Beuselinck and colleagues, Catholic University of Leuven, wrote, "Linearity of extraction was tested on dilution series of HCV and HBV reference...

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Source: Blood Weekly (2006-01-12)

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