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Diagnostics

Low serotonin levels in plasma accurately measured by novel HPLC assay

Published in Blood Weekly, April 27th, 2006

A novel HPLC assay provides an accurate measure of low serotonin levels in plasma.

Scientists in England devised a "sensitive and rapid assay" for "the measurement of low concentrations of 5-hydroxytryptamine (5-HT) present in human platelet-depleted plasma (PDP) using reverse-phase high performance liquid chromatography (HPLC) with fluorimetric detection."

"With an analysis time of 12 minutes, this method is particularly useful for large-scale clinical trials investigating small differences in PDP 5-HT concentrations in conditions such as functional gastrointestinal disorders (FGID)," noted W. Atkinson and coauthors at South Manchester University...

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