Chromatography

Reports outline chromatography study results from M. Wojciakkosior et al

Published in Blood Weekly, February 21st, 2008

"A method based on high performance thin-layer chromatography combined with densitometry for the simultaneous determination of oleanolic and ursolic acids is described. Because of the similarity of chemical structure the prechromatographic derivatization was necessary to separate these triterpenic acids," investigators in Lublin, Poland report.

"The samples were treated by 1 % iodine solution in chloroform directly on the chromatographic plate and developed with the mobile phase consisting of A (petroleum ether), B (ethyl acetate) and C (acetone) (8.2:1.8:0.1, v/v/v). After drying, the plates were sprayed with 10% (v/v) ethanol solution of sulfuric acid(VI) and heated...

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Source: Blood Weekly (2008-02-21)

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