"Activation of Methotrexate Phenylalanine by Monoclonal Antibody - Carboxypeptidase A Conjugate for the Specific Treatment of Ovarian Cancer In Vitro."

According to the authors' abstract of an article published in British Journal of Cancer, "Monoclonal antibody 4E3 directed against a glycosylated surface protein on human ovarian teratocarcinoma cells (CRL-1572 cell line) was conjugated to bovine carboxypeptidase A (CPA) using a 3400 Da polyethylene glycol chain bearing an N-hydroxysuccinimide group at both ends. The conjugate preparation was purified by fast protein liquid chromatography on a Superose 12/30 HR column. The 4E3-CPA conjugate was recovered in the third fraction by SDS-PAGE analysis. The specific binding of the 4E3-CPA conjugate to CRL-1572 cells was confirmed by a FAGS analysis and the enzymatic activity of the conjugate...

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