"Laboratory Preparation of a Deglycosylated Ricin Toxin A Chain Containing Immunotoxin Directed Against a CD7 T Lineage Differentiation Antigen for Phase I Human Clinical Studies Involving T Cell Malignancies."

According to the authors' abstract of an article published in Journal of Immunological Methods, "An immunotoxin consisting of a monoclonal antibody specific for CD7, a cell surface determinant expressed on T acute lymphocytic leukemia (T-ALL) blast cells, was linked to the potent plant toxin deglycosylated ricin toxin A chain (dgRTA) and is currently under evaluation in Phase I clinical trials. Scale-up production of this immunotoxin, called DA7, was simplified using a two-step purification protocol that resulted in a highly purified immunotoxin meeting FDA criteria for IND approval. The anti-CD7 antibody, 3A1e, an IgG2b, was coupled to toxin using two different heterobifunctional...

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