"In Vitro Studies of Tumor Cell Transfection with Plasmid DNA in a Cationic Lipid Vector."

According to an abstract submitted by the authors to the Fifth International Conference on Gene Therapy of Cancer, held November 14-16, 1996, in San Diego, California, "A series of in vitro studies were done to characterize, optimize and define the limits of efficiency of transfection of human tumors with genes delivered as plasmids in cationic lipids. The hypothesis is that efficient, relatively long lasting gene transfer without the requirement for prolonged culture and selection would be useful for the generation of gene-modified tumor cell vaccines. The lipid was DMRIE/DOPE and the cells were transfected at a lipid/DNA ratio of 1:1 using 10.0 (mu)g/ml DNA. The plasmids included LacZ...

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