Cancer Gene Therapy
"A Comparison of Gene Transfer Methods in Human Dendritic Cells."
Published in Cancer Weekly, March 10th, 1997
According to the authors' abstract of an article published in Cancer Gene Therapy, "Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) for the initiation of antigen-specific T-cell activation. DCs may be highly enriched from peripheral blood-adherent leukocytes by short-term (7-day) culture in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor. Various methods of gene transfer were studied, including DNA/liposome complexes, electroporation, CaPO4 precipitation, and recombinant adenovirus (AdV) vectors. Low levels of expression were obtained with the physical methods tested. In contrast, AdV vectors expressing luciferase,...
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Source: Cancer Weekly (1997-03-10)
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