"Direct in vivo Measurement of Targeted Binding in a Human Tumor Xenograft."

According to the authors' abstract of an article published in Proceedings of the National Academy of Sciences of the United States of America, "Binding is crucial to the function of most biologically active molecules, but difficult to quantify directly in living tissue. To this end, fluorescence recovery after photobleaching was used to detect the immobilization of fluorescently labeled ligand caused by binding to receptors in vivo. Measurements of mAb affinity to target antigen within human tumor xenografts revealed a saturable binding isotherm, from which an in vivo carcinoembryonic antigen density of 0.56 nmol/g (5.0x10⁵/cell) and an association constant of K-a less than or...

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