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Cancer Histochemistry

Hue-Saturation-Intensity (HSI) works better than the gold standard

Published in Cancer Weekly, May 6th, 2003

Histochemical staining of lung cancer tissue by hue saturation density for levels of proliferating cells with Ki-67 worked better than the gold standard.

"We tested a recently developed flexible method of separation and quantification of immunohistochemical staining by means of color image analysis. An algorithm was recently developed to deconvolve the color information acquired with RGB cameras, to calculate the contribution of each of the applied stains, based on the stain-specific RGB absorption," scientists in the United States report.

"The algorithm was tested using a set of lung-tumor samples labeled for the detection of Ki-67, an antigen...

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