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Cervical Cancer

PCR more HPV-sensitive than signal amplified in situ hybridization

Published in Cancer Weekly, October 19th, 2004

According to a report from Austria, "Archival paraffin-embedded tumor specimens offer a wealth of information for both cancer research and for routine clinical applications.

"However, the use of formalin-fixed, paraffin-embedded specimens for quantitative real-time polymerase chain reaction (PCR) is not yet a standard diagnostic method in many laboratories, in particular for the quantification of human papillomavirus (HPV). Particularly high-risk HPV types are involved in almost 100% of the carcinogenesis of cervical cancer," wrote K. Biedermann and colleagues.

They "compared the diagnostic applicability and sensitivity of real-time PCR to that of...

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