Embryonic Stem Cell Research
More embryos reach blastocyte stage in vitro without leukemia inhibitory factor
Published in Cancer Weekly, June 7th, 2005
"In vitro produced (IVP) bovine embryos were subjected to in vitro culture with or without 1000 U/ml human recombinant leukemia inhibitory factor (LIF) added to the culture medium from Days 5 to 8 post insemination (p.i.). Resulting blastocysts were subsequently plated intact on mouse feeder cells in a medium with or without LIF.
"Significantly more embryos reached the hatched blastocyst stage, and the number of blastocysts with excellent morphology was significantly higher, when LIF was omitted. At Day 8 p.i., total cell count (TCC) and inner cell...
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Source: Cancer Weekly (2005-06-07)
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