Published in Cancer Weekly, August 15th, 2006
"A preparative counter-current chromatography (CCC) was used to isolate and separate chemical constituents from the leaf of P. villosa, a famous traditional Chinese medicinal herb. Six flavonoids including 2 known and 4 novel compounds were successfully simultaneous purified by CCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (10:13: 13:10, v/v) by increasing the flow rate of the mobile phase from 1.0 ml/min to 2.0 ml/min after 110 min to bring out the late eluters," investigators in the People's Republic of China...
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