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Cancer Gene Therapy
Studies from Shandong University in the area of cancer gene therapy published
May 6th, 2008
According to a study from Jinan, People's Republic of China, " To investigate the inhibitory effect of retrovirus-mediated antisense human telomerase RNA (hTR) gene therapy on hepatocelluar carcinoma. We first constructed the sense and antisense hTR vectors and then transfected these into HepG2 cells." "Telomerase activity, cell growth curves, proliferating cell nuclear antigen expression (PCNA), cell cycle distribution, and cell apoptosis were detected by the means of telemere repeat amplification protocol (TRAP), MTT assay, immunofluorescence, flow cytometric analysis, and transferase-mediated nick end labeling (TUNEL), respectively. In order to further confirm the...
Source: Cancer Weekly (2008-05-06)
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