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Angiogenesis
Data on angiogenesis discussed by J. Wu and colleagues
June 3rd, 2008
According to recent research from Guangzhou, People's Republic of China, "Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody." "Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hybridoma cells stably and specifically binds to angiogenin. The fused protein angiogenin-EGF was then expressed in HUVECs, and the subcellular localization of the recombinant protein was determined by confocal microscopy and TEM assay," wrote J. Wu and colleagues. ...
Source: Cancer Weekly (2008-06-03)
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