Gene Therapy
Research on gene therapy published by scientists at Hoshi University
Published in Cancer Weekly, May 5th, 2009
According to recent research from Ebara, Japan, "As we have previously reported the delivery of plasmid DNA (DNA) complexed with oligoarginine-PEG artificial lipids (oligoarginine/DNA complexes). we focused on tetra- and decaarginine (Arg4, Arg10) to improve transfection efficiency by both the formation of oligoarginine-coated DNA complexed with protamine (PD), and the addition of Ca2+ after formation of complexes. The efficiency of DNA condensation was determined by gel electrophoresis."
"Cellular uptake and transfection efficiency were evaluated in human cervical carcinoma HeLa cells using flow cytometry and luciferase assay. Oligoarginine-coated PD enhanced...
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Source: Cancer Weekly (2009-05-05)
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