Artificial Chromosomes
Findings from H.Y. Cui et al in artificial chromosomes reported
Published in Cancer Weekly, May 19th, 2009
According to recent research published in the Journal of Virological Methods, "Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphoma in poultry. The complete genome of the avirulent vaccine strain MDV-814 was cloned as an infectious bacterial artificial chromosome (BAC) using an 8.8-kb fragment containing the self-designed selective marker guanosine phosphoriboxyl transferase."
"The recombinant virus MDV-814-BAC was generated by co-transfection of a BAC transfer vector and MDV-814 total DNA, and was purified by eight rounds of selective passaging. The infectivity of the BAC DNA clone was validated by MDV...
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Source: Cancer Weekly (2009-05-19)
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