Hepatocellular Cancer
Researchers from Nanjing Normal University provide details of new studies and findings in the area of hepatocellular cancer
Published in Drug Law Weekly, September 22nd, 2009
"The aim of this study was to construct a ribosome display library of single chain variable fragments (scFvs) associated with hepatocarcinoma and screen such a library for hepatocarcinoma-binding scFvs. mRNA was isolated from the spleens of mice immunized with hepatocellular carcinoma cell line HepG2. Heavy and k chain genes (VH and k) were amplified separately by RT-PCR, and an anti-HepG2 VH/k chain ribosome display library was constructed by assembling VH and k into the VH/k chain with a specially constructed linker by SOE-PCR," researchers in Nanjing, People's Republic of China report.
"The VH/k chain library was transcribed and translated in vitro using a rabbit...
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Source: Drug Law Weekly (2009-09-22)
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