Pancreas

Recent findings from Semmelweis University highlight research in pancreas

Published in Drug Week, January 1st, 2010

"The human pancreatic duct cell line, HPAF, has been shown previously to secrete Cl-in response to Ca2+-mobilizing stimuli. Our aim was to assess the capacity of HPAF cells to transport and secrete HCO3-," scientists in Budapest, Hungary report.

"HPAF cells were grown as confluent monolayers on permeable supports. Short-circuit current was measured by voltage clamp. Intracellular pH (pH(i)) was measured by microfluorometry in cells loaded with 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). In HCO3--free solutions, ATP-evoked changes in short-circuit current were inhibited by bumetanide, and the recovery of pHi from acid loading was abolished by...

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Source: Drug Week (2010-01-01)

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