Blood Proteins

Data on blood proteins detailed by researchers at Osaka Medical College, Medical College

Published in Drug Week, October 15th, 2010

"In clinical settings, human serum albumin (HSA) is widely quantitated on the basis of change in the color of bromocresol purple (BCP) bound to HSA. However, the binding sites of BCP on HSA are hitherto unknown," scientists writing in the journal Clinica Chimica Acta report.

"We have identified these sites by performing binding experiments in which BCP was bound to HSA complexed with warfarin, ibuprofen, propofol, or myristic acid. The binding experiment was performed with HSA complexed with a drug in buffers at pH 4.8 and 7.2. The number of BCP binding sites (n) and the association constant (Ka) were obtained by Scatchard analysis using the absorbance data of...

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Source: Drug Week (2010-10-15)

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