Cardiology
Findings from University of Alabama Provide New Insights into Cardiology
Published in Drug Week, July 29th, 2011
2011 JUL 29 -- "We developed a new method for ratiometric optical mapping of transmembrane potential (V-m) in cardiac preparations stainedwith di-4-ANEPPS. V-m-dependent shifts of excitation and emission spectra establish two excitation bands (<481 and >481 nm) that produce fluorescence changes of opposite polarity within a single emission band (575-620 nm)," scientists in Birmingham, Alabama report.
"The ratio of these positive and negative fluorescence signals (excitation ratiometry) increases V-m sensitivity and removes artifacts common to both signals. We pulsed blue (450 +/- 10 nm) and cyan (505 +/- 15 nm) light emitting diodes (LEDs) at 375 Hz...
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Source: Drug Week (2011-07-29)
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