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Fitness & Wellness Business Week

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Enzyme Research



Scientists at Ataturk University publish research in enzyme research



August 20th, 2008

According to a study from Erzurum, Turkey, "Carbonic anhydrase (CA) enzyme was purified from rainbow trout brain by Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography. The enzyme was obtained with a specific activity of 2,275 EU mg(-1) and a yield of 22.5%."

"The sample obtained from the affinity column was used for kinetic properties and inhibition studies. Both optimum and stable pH were found as 9.0 in 1 M Tris-SO4 at 4 degrees C, respectively. To check the purity and subunit molecular weight of enzyme, sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis was performed, and MW was found as approximately 29.0 kDa. The molecular weight of native...


Source: Fitness & Wellness Business Week (2008-08-20)

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