Cancer/Gene Therapy

Team Develops Method To Regenerate Virus from Vectors

Published in Gene Therapy Weekly, February 13th, 1995

A team of American researchers has used the retroviral vector pDOL and an oncogenically activated form of c-Ha-ras to develop a means of rapidly regenerating virus from a colony of cells infected with a retroviral vector and of establishing a producer line of new virus.

Retroviral vectors offer a reliable alternative to transfection and electroporation as a means to introduce foreign genes into cells in vitro and have shown promise for the application of gene therapy in vivo. Normally, retroviral vectors infect a packaging cell line, which then infects the target cells, and cannot reproduce. In some instances, however, progeny of the retroviral vectors may be...

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Source: Gene Therapy Weekly (1995-02-13)

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