"Efficient Transduction of CD34⁺ Cells with an Anti-HIV-1 Ribozyme-Bearing Retroviral Vector."

According to an abstract submitted by the authors to the 36th Annual Meeting of the American Society of Hematology, held December 2-6, 1994, in Nashville, Tennessee, "Previous experiments have demonstrated an efficient gene transfer of neomycin phosphotransferase (neo) in LNL-6 vector into blood-derived CD34⁺ cells mobilized by growth factors. Even though the transgene can be found by DNA PCR in 67-100% cells with clonogenic activity, only about 10% of the colonies were positive for gene expression as determined by RNA PCR. As the reporter gene neo used was under the control of the LTR promoter alone, we have used retroviral vector constructs containing a ribozyme gene driven...

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