"Construction of Plasmid Vectors to Produce Antisense RNA in Mycobacteria."

According to an abstract presented by the authors to the Molecular Mechanisms in Tuberculosis Keystone Symposium, held February 19-25, 1995, in Tamarron, Colorado, "A key step in the analysis of a cloned gene is to elucidate its biologic function in the native organism. Classically, this has been done by constructing a 'knock-out' mutant by site-directed mutagenesis of the cloned gene followed by replacement of the wild-type gene with the mutated gene by homologous recombination. Unfortunately, even with the recent advances in the study of Mycobacterium tuberculosis, reliable homologous recombination has not yet been achieved. An alternative approach to gene replacement for...

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