"High Efficiency Gene Modification of Primary Tumor, Effector CD8⁺ T Lymphocytes and CD34⁺ Cells Using Adeno-Associated Virus Plasmid DNA:Liposom

According to an abstract submitted by the authors to the 86th Annual Meeting of the American Association for Cancer Research, held March 18-22, 1995, Toronto, Ontario, Canada, "Strategies including gene modification of primary tumor cells for vaccination, effector T lymphocytes and hematopoietic stem cells depend in large part upon the development of vector systems to efficiently introduce DNA into these cells. We have used cationic liposomes to facilitate plasmid DNA containing the inverted terminal repeats (ITR) of adenoassociated virus (AAV) for transfection of primary cell types. We have investigated expression of reporter gene constructs such as chloramphenicol acetyl transferase...

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