"E- Vectors: Development of Novel Self-Inactivating and Self-Activating Retroviral Vectors for Safer Gene Therapy."

According to the authors' abstract of an article published in Journal of Virology, "We have developed novel self-inactivating and self-activating retroviral vectors based on the previously observed high-frequency deletion of direct repeats. We constructed spleen necrosis virus (SNV)-based viral vectors that contained large direct repeats flanking the viral encapsidation sequence (E). A large proportion of the proviruses in the target cells had E and one copy of the direct repeat deleted. Direct repeats of 1,333 and 788 bp were deleted at frequencies of 93 and 85%, respectively. To achieve a 100% deletion efficiency in target cells after ex vivo infection and drug selection, we...

Want to see the full article?

• Purchase this article for only $3.00