Immunology
"Leishmania major: Immunogenicity and Antigenicity of a gp63 Fused to the Oprl Lipoprotein of Pseudomonas aeruginosa."
Published in Gene Therapy Weekly, February 5th, 1996
According to an abstract submitted by the authors to the European Conference on Tropical Medicine, held October 22-26, 1995, in Hamburg, Germany, "The major surface glycoprotein gp63 of Leishmania major contained in a 2.2 Kb fragment was subcloned into the lipoprotein expression vector pVUB2. The gp63 was induced and expressed as fusion protein with the Pseudomonas aeruginosa lipoprotein Oprl. Under reducing conditions on SDS-PAGE the Oprl p63 fusion protein was seen as a large fusion protein of 79,000 Kd. Large amounts of Oprl-gp63 hybrid protein was fully translocated into the outer membrane of E. coli mediated by the signal peptide sequence of the...
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Source: Gene Therapy Weekly (1996-02-05)
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