"Gene Delivery into the Renal Glomerulus by Transfer of Genetically Engineered, Autologous Mesangial Cells."

According to the authors' abstract of an article published in Experimental Nephrology, "To obviate the problem of rejection in situations where cells are used as vectors for gene delivery, the feasibility of using autologous mesangial cells cultured from renal biopsy specimens was studied for the purpose of gene transfer into the glomerulus. Using the calcium-phosphate co-precipitation method, a reporter gene which encodes bacterial beta-galactosidase was introduced into cultured mesangial cells derived from renal biopsy tissue of rats. Stable transfectants were established in the presence of a selection drug and then transferred back into the contralateral kidneys of the same animals...

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