Hematopoietic Stem Cells
"High-Frequency Cell Surface Expression of a Foreign Protein in Murine Hematopoietic Stem Cells using a New Retroviral Vector."
Published in Gene Therapy Weekly, March 4th, 1996
According to the authors' abstract of an article published in Blood, "A retroviral vector (pSFF) derived from murine Friend spleen focus forming virus was used to transduce murine hematopoietic stem cells and express a cell surface marker protein, mutated murine prion protein, in vitro and in vivo after transplantation. To enhance retroviral vector integration in bone marrow cells, mice were treated with 5-fluorouracil (5-FU) to increase stem cell mitotic activity, which peaked on day 8 post-5-FU. The infectivity titer of the vector, pSFF-mPrP-3F4, was determined by a novel assay in which antigen-positive foci of infected cells were detected after replication and spread of the vector in...
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Source: Gene Therapy Weekly (1996-03-04)
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