Vectors
Bicistronic Therapeutic Retroviral Vector Enables Cell Sorting
Published in Gene Therapy Weekly, March 18th, 1996
Inefficiencies associated with the use of retroviral vectors for transfecting hematopoietic cells have stimulated the development of methods to increase their usefulness. Sorting transfected cells from non-transfected cells in order to deliver a higher percentage of therapeutic cells to the patient is one method.
Sorting methods have focused on marker genes or genes that confer drug resistance to the transduced cell. Both of these procedures are promising. However, the genes used to date for sorting have been relatively large, which...
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Source: Gene Therapy Weekly (1996-03-18)
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