"Enhanced Activity of an Antisense Oligonucleotide Targeting Murine Protein Kinase C-Alpha by the Incorporation of 2'-O-Propyl Modifications."

According to the authors' abstract of an article published in Nucleic Acids Research, "We have previously described the characterization of a 20mer phosphorothioate oligodeoxynucleotide (ISIS 4189) which inhibits murine protein kinase C-alpha (PKC-alpha) gene expression, both in vitro and in vivo. In an effort to increase the antisense activity of this oligonucleotide, 2'-O-propyl modifications have been incorporated into the 5'- and 3'-ends of the oligonucleotide, with the eight central bases left as phosphorothioate oligodeoxynucleotides. Hybridization analysis demonstrated that these modifications increased affinity by similar to 8 and 6(degree)C per oligonucleotide for the...

Want to see the full article?

• Purchase this article for only $3.00