"Complementation of a Primer Binding Site-Impaired Murine Leukemia Virus-Derived Retroviral Vector by a Genetically Engineered tRNA-Like Primer."

According to the authors' abstract of an article published in Journal of Virology, "Reverse transcription of retroviral genomes is primed by a tRNA annealed to an 18-nucleotide primer binding site. Here, we present a primer complementation system to study molecular interaction of the replication machinery with the primer and primer binding site in vivo. Introduction of eight base substitutions into the primer binding site of a murine leukemia virus-based vector allowed efficient RNA encapsidation but resulted in severely reduced vector replication capacity. Replication was restored upon complementation with a synthetic gene designed to encode a complementary tRNA-like primer, but not...

Want to see the full article?

• Purchase this article for only $3.00