Vaccine Development (TB)
"Purification and Immunological Characterization of 12 Recombinant Culture Filtrate Proteins of Mycobacterium tuberculosis."
Published in Gene Therapy Weekly, May 26th, 1997
According to an abstract submitted by the authors to the 97th General Meeting of the American Society for Microbiology, held May 4-8, 1997, in Miami Beach, Florida, "Proteins that are actively secreted by M. tuberculosis are potent inducers of immune responses during TB. To characterize the immunobiological properties of individual proteins found in the M. tuberculosis culture filtrate (CF), we have expressed and purified CF proteins from E. coli cells. In this paper we describe protocols used to purify twelve recombinant CF proteins of M. tuberculosis in an immunologically active form and free from E. coli lipopolysaccharide (LPS), a potent polyclonal...
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Source: Gene Therapy Weekly (1997-05-26)
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