"A Novel Fluorescent Tag for Labelling of Some Antisense Oligonucleotides."

According to the authors' abstract of an article published in Neurochemistry International, "A new versatile fluorescent tag, 4-acetylamino-1,8-naphthalimido-N-caproic acid (NCA) has been synthesized and characterized, and the optimal conditions for the maximum fluorescence using this tag allow a sensitivity level of 10^-9 mol/1. Useful fluorescence is observed upon covalent linking of this tag via its activated carboxy end to an alkylamino moiety, activated by the use of carbonyldiimidazole and hexamethylenediamine at the 5'-termini of an octamer, d(TCTTGCTC), a 33-mer, and a 41-mer complementary to the tar initiator of the HIV-1 genome. A new method for the generation of...

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