Gene Transfer
"Cell Cycle Analysis of Retroviral Vector Gene Expression During Early Infection."
Published in Gene Therapy Weekly, May 18th, 1998
According to the authors' abstract of an article published in Cytometry, "To examine the pattern of retroviral vector gene expression during early stages of infection, a Moloney murine leukemia virus (M-MuLV)-based vector that transcribes the simian virus 40 (SV40) large T antigen (Tag) gene from the viral long terminal repeat (LTR) was used to infect proliferating rodent fibroblasts. At various times after infection, cells were fixed and stained for Tag by indirect immunofluorescence, and for DNA using propidium iodide, Tag immunofluorescence and DNA content were both quantified by flow cytometry. The results showed that Tag expression was first detected exclusively in the late G(1) and...
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Source: Gene Therapy Weekly (1998-05-18)
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