"Adenovirus-Mediated Transfer of a Modified Human Proinsulin Gene Reverses Hyperglycemia in Diabetic Mice."

According to the authors' abstract of an article published in American Journal of Physiology - Endocrinology and Metabolism, "The human proinsulin cDNA was introduced into a replication-defective adenovirus and was found to confer proinsulin expression to a hepatocyte (H4-II-E) cell line upon infection. A second virus was constructed in which the dibasic prohormone convertase recognition sequence was mutated to a tetrabasic furin cleavage site. Cells infected with this virus synthesized both proinsulin and mature insulin. Gel filtration chromatography, competition of insulin binding, and activation of the insulin receptor kinase activity demonstrated that this mature insulin was...

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