"High-Efficiency Gene Transfer Into Skeletal Muscle Mediated by Electric Pulses."

According to the authors' abstract of an article published in Proceedings of the National Academy of Sciences of the United States of America, "Gene delivery to skeletal muscle is a promising strategy for the treatment of muscle disorders and for the systemic secretion of therapeutic proteins. However, present DNA delivery technologies have to be improved with regard to both the level of expression and interindividual variability. We report very efficient plasmid DNA transfer in muscle fibers by using square-wave electric pulses of low field strength (less than 300 V/cm) and of long duration (more than 1 ms). Contrary to the electropermeabilization-induced uptake of small molecules into...

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