Vector Development

Lipoplexes Should Be Physiochemically Characterized

Published in Gene Therapy Weekly, September 27th, 1999

The lamellarity of cationic liposomes and the mode of preparation of lipoplexes affect their transfection ability in NIH-3T3 cells.

N.J. Zuidam and colleagues from the Hebrew University of Jerusalem, Israel, studied transfection of NIH-3T3 cells by a human growth hormone expression vector complexed with liposomes composed of N-(1-(2,3-dioleoyloxy)propyl)-N,N,N-trimethylammonium chloride (DOTAP) with or without helper lipids. Their study results were published in the journal Biochimica Et Biophysica Acta - Biomembranes ("Lamellarity of Cationic Liposomes and Mode of Preparation of Lipoplexes Affect Transfection Efficiency," Biochimica Et Biophysica Acta - Biomembranes,...

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Source: Gene Therapy Weekly (1999-09-27)

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