Published in Gene Therapy Weekly, April 12th, 2001
R. Youil and colleagues devised a procedure that allows isolation of recombinant adenoviral genomes that are safe for human use in genetic therapy. They published the protocol in the March 2001 issue of the Journal of Virological Methods.
"The procedure uses a bacterial homologous recombination system and results in the conversion of the double-stranded linear adenovirus genome into a circularized plasmid form that can be easily analyzed by restriction...
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