Histology

Researchers Develop Novel Method For Transgene Detection

Published in Gene Therapy Weekly, May 24th, 2001

by Deborah W. Heinrich, PhD, staff medical writer - Currently used methods for detection of transgenes in histological samples require a specified minimum of DNA to be present in order for the methods to work. Common gene therapy vectors maintain extremely low copy numbers, making detection by conventional methods difficult, explained researchers at the University of Kuopio in Finland.

In response, P.M. Leppanen and colleagues developed a novel protocol designed to detect small amounts of target DNA in paraffin-embedded and frozen tissue sections. The new method is called in situ polymerase chain reaction (in situ PCR), and they explained the procedure in the...

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Source: Gene Therapy Weekly (2001-05-24)

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