Technology

A Novel Protocol Uses Flow Cytometry To Quantify Gene Delivery

Published in Gene Therapy Weekly, August 9th, 2001

by Deborah W. Heinrich, PhD, staff medical writer - Using artificial chromosome expression systems (ACes), researchers in British Columbia have devised a novel screening approach to detecting gene delivery.

S. Vanderbyl and coworkers, Chromos Molecular Systems, Inc., Burnaby, B.C., Canada, set the goal of quantifying gene transfer after gene delivery via ACes. They wanted their system to detect heterologous DNA after transfer via other methods as well. Thus they created a protocol in which artificial chromosomes were labeled with a thymidine analogue. The analogue was detected by flow cytometry in vitro.

Chinese hamster ovary (CHO) cells harboring...

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Source: Gene Therapy Weekly (2001-08-09)

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