Published in Gene Therapy Weekly, August 9th, 2001
S. Vanderbyl and coworkers, Chromos Molecular Systems, Inc., Burnaby, B.C., Canada, set the goal of quantifying gene transfer after gene delivery via ACes. They wanted their system to detect heterologous DNA after transfer via other methods as well. Thus they created a protocol in which artificial chromosomes were labeled with a thymidine analogue. The analogue was detected by flow cytometry in vitro.
Chinese hamster ovary (CHO) cells harboring...
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