Transplantation Medicine

Ex Vivo Gene Transfer Effects Corneal Graft Survival

Published in Gene Therapy Weekly, August 9th, 2001

by Deborah W. Heinrich, PhD, staff medical writer - Researchers demonstrated the effects of adenovirus-mediated cytokine expression on the corneal graft survival.

S.A. Rayner and colleagues at the University of London Imperial College of Science Technology and Medicine in London, U.K., hoped to extend corneal graft survival by expressing tumor necrosis factor receptor (TNFR) in corneal tissues. For this purpose, they constructed an adenovirus vector expressing a TNFR/immunoglobulin fusion protein (AdTNFR). Rabbit corneal tissue was transfected with AdTNFR ex vivo and expression was confirmed with enzyme-linked immunosorbent assay and bioassay. Ex vivo,...

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Source: Gene Therapy Weekly (2001-08-09)

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