Published in Gene Therapy Weekly, August 23rd, 2001
M.A. Perricone and colleagues, Genzyme Corp., constructed a recombinant adenovirus vector expressing the cystic fibrosis trans-membrane regulator (Ad2/CFTR), which they administered to cystic fibrosis (CF) patients. Airway epithelial cells were evaluated for transfection by polymerase chain reaction (PCR) and CFTR expression by reverse transcriptase PCR (rtPCR). Transfection efficiency was evaluated by...
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